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Histochemical aspects of wheat resistance to leaf blast mediated by silicon Scientia Agricola
Silva,Washington Luís da; Cruz,Maria Fernanda Antunes; Fortunato,Alessandro Antônio; Rodrigues,Fabrício Ávila.
Blast, caused by Pyricularia oryzae, has become a significant disease threat to wheat (Triticum aestivum L.) in Brazil. This study aimed to investigate at the histochemical level if silicon (Si) could enhance the production of flavonoids in the leaves of wheat plants in response to P. oryzae infection. Plants from the Aliança cultivar, which are susceptible to blast, were grown in hydroponic cultures containing 0 (-Si) or 2 mM of Si (+Si) and inoculated by spraying a conidial suspension of P. oryzae (1 × 105 conidia mL−1) on all adaxial leaf surfaces of plants at 60 days after emergence (growth stage 65). The fourth and fifth leaves of each plant were used to evaluate blast severity at 24, 36, 48, 72 and 96 h after inoculation (hai). At 96 hai, leaves were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyricularia oryzae; Flavonoids; Histochemistry; Phenolics.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400322
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Histochemical changes during the ontogeny of malagueta and biquinho pepper seeds Agronomy
Abud, Haynna Fernandes; Araújo, Eduardo Fontes; Araujo, Roberto Fontes; Picoli, Edgard Augusto de Toledo; Gallão, Maria Izabel.
This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol’s iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsicum frutescens; C. chinense; Chemical composition; Histochemistry; Reserves..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32492
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Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion International Journal of Morphology
Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S..
The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide +...
Tipo: Journal article Palavras-chave: Rat; Otic ganglion; Autonomic nervous system; Histochemistry.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015
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Histological and Histochemical Changes by Clove Essential Oil Upon the Gonads of Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) International Journal of Morphology
Cruz,Glaucilane dos Santos; Teixeira,Valeria Wanderley; de Oliveira,Jose Vargas; Teixeira,Alvaro Aguiar Coelho; Araújo,Alicely Correia; Alves,Thiago Jose de Souza; da Cunha,Franklin Magliano; Breda,Mariana Oliveira.
Spodoptera frugiperda is a polyphagous insect that causes economic losses to several crops in Brazil and is the major obstacle to corn production. Researches focusing on alternative control, e.g. botanical products are expanding to offer a wide variety of molecules that interfere with different biological parameters of insect pests. Thus, this study tested the hypothesis that clove essential oil affects the spermatogenesis, ovarioles histochemistry and the fertility of S. frugiperda. The results showed that clove essential oil affects the gametogenesis of S. frugiperda ovarioles, reflecting negatively on its reproduction, proving to be a promising tool for controlling this pest.
Tipo: Journal article Palavras-chave: Fall armyworm; Spodoptera frugiperda; Reproduction; Histochemistry; Histology; Syzygium aromaticum.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400034
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Histological and Histochemical Studies of the Aorta and Pulmonary Trunk in STZ-induced Diabetic Wistar Rats Treated with Momordica charantia International Journal of Morphology
Komolafe,O. A; Ofusori,D. A; Adewole,O. S; Ayoka,A. O; Bejide,R.
Diabetes mellitus (DM) is a serious metabolic disorder with micro and macro-vascular complications that result in a significant morbidity and mortality. The present study investigated the effects of Momordica charantia (M. charantia) on histological changes of the aorta and pulmonary trunk in streptozotocin-induced diabetic Wistar rats. Forty healthy adult Wistar rats of both sexes were randomly assigned into five groups A, B, C, D and E of eight rats each. Group A were the control (normal rats); B were the experimentally-induced diabetic rats; C were diabetic rats treated with methanolic extracts of M. charantia for two weeks (withdrawal group); D were diabetic rats treated with methanolic extracts of M. charantia for four weeks. E was diabetic rats...
Tipo: Journal article Palavras-chave: Diabetes; Momordica charantia; Histological; Histochemistry; Aorta; Pulmonary trunk.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200060
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Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010
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Histopathology of Organs from Neonate Offspring Born to Female Rats Exposed to Sublethal Doses of Biological and Synthetic Insecticides International Journal of Morphology
dos Santos,Hilda Michelly Paiva; Teixeira,Valéria Wanderley; Teixeira,Álvaro Aguiar Coelho; da Silva,Rosimere; Lemos,Ana Janaina Jeanine Martins; Neto,Clovis José Cavalcanti Lapa; Ferreira,Cintia Giselle Martins.
The current annual consumption of insecticides in agriculture in Brazil is over three million tons. The exposure happens through food that is contaminated with toxic waste and respiratory and dermal absorption. Biological control with Bacillus thuringiensis Berliner (Bt) has assumed increasing importance replacing the use of conventional insecticides. However, the tests used to justify approval for use and market release of Bt products, and the protocols used to assess the development of toxicity, are extremely superficial. In addition, more studies comparing effects between males and females and during pregnancy and lactation have not been conducted. Thus, the hypothesis tested in this study was if the administration of the XenTari® WG (Bacillus...
Tipo: Journal article Palavras-chave: Bacillus thuringiensis; Synthetic insecticide; Rats; Histology; Histochemistry.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100040
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Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions International Journal of Morphology
Rosero-Salazar,Doris Haydee.
Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using...
Tipo: Journal article Palavras-chave: Histochemistry; Ischemia; Reperfusion; Image analysis; Segmentation; Histoquímica; Isquemia; Reperfusión; Análisis de imágenes; Segmentación.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019
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Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) International Journal of Morphology
Longo,María Victoria; Díaz,Alcira Ofelia.
The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to 'extreme' groups, whereas types II and III would be considered 'intermediate'. Type I fibers were large and exhibited a weak reaction...
Tipo: Journal article Palavras-chave: Euryhaline crabs; Histochemistry; Hyper regulation; Muscle fibers; Salinity stress.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200025
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Leaf anatomy and histochemistry of Macropeplus dentatus (Perkins) I. Santos & Peixoto and Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae) Acta Botanica
Costa,Fernanda Flores; Lima,Helena Regina Pinto; Cunha,Maura Da; Santos,Inês da Silva.
The Monimiaceae is represented in the Neotropical region by six genera. Macropeplus Perkins is a Brazilian genus comprising four species restricted to high-montane Atlantic Forest areas, which have not been properly examined from the anatomical point of view. This study characterizes the anatomical structure and histochemical aspects of the leaves of Macropeplus dentatus and M. ligustrinus, and adds to our taxonomic knowledge ofthe genus. The individuals studied were collected in the Serra dos Órgãos National Park and in the Caraça Natural Park in southeastern Brazil. Among the characteristics common to the two species, hypostomatic leaves, dorsiventral structure, paracytic stomata, a subepidermal layer, collateral vascular system in the form of an arc in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Rain Forest; Histochemistry; Leaf anatomy; Macropeplus; Monimiaceae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000300027
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Leaf glands of Banisteriopsis muricata (Malpighiaceae): distribution, secretion composition, anatomy and relationship to visitors Acta Botanica
Nery,Lays Araújo; Vieira,Milene Faria; Ventrella,Marília Contin.
ABSTRACT Leaf glands are common structures in Malpighiaceae and exhibit great morphological diversity, yet information on their anatomy, secretion and type of visitors remains scarce. The aim of this study was to describe the distribution, anatomical development and chemical and functional properties of leaf glands of Banisteriopsis muricata (Malpighiaceae). Leaves at different stages of development were collected and processed according to standard techniques for light and scanning electron microscopy. Secretion composition was determined by histochemical tests and test-strips, while gland funciton was determined by field observation of interactions with visitors. Leaf glands were located on the petiole and on the abaxial base of the leaf blade. The gland...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Ants; Extrafloral nectaries (EFNs); Histochemistry; Leaf glands; Nectar; Ontogeny.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300459
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Light exercise causes adaptive changes in muscles of young Brasileiro de Hipismo horses Ciência Rural
D'Angelis,Flora Helena de Freitas; Silva,Marco Augusto Giannoccaro da; Martins,Carla Braga; Ferraz,Guilherme de Camargo; Oliveira,João Ademir de; Boleli,Isabel Cristina; Lacerda-Neto,José Corrêa de; Queiroz-Neto,Antonio de.
This study aimed to determine the changes in the Gluteus medius of 4 year-old Brasileiro de Hipismo (BH) horses submitted to light physical activity for 10 months. The study involved 11 horses from the "Nove de Julho" Battalion of the Military Police of São Paulo State (PM-SP). Percutaneous muscle biopsy was performed in horses at maintenance and in those that had participated in routine police work for 10 months with the PM-SP. Fragments of the left Gluteus medius muscle was removed at depths of 20mm and 60mm. To determine the fiber types, the histochemical analyses were performed for myofibrillar adenosine triphosphatase (mATPase) and nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR). The morphometry of the fibers was performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brasileiro de Hipismo breed; Horse; Histochemistry; Gluteus medius muscle; Low intensity exercise.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000500017
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Localisation immunohisto chimique de peptides apparentes aux hormones de vertebres chez Pecten maximus ArchiMer
Donval, A.
Substances related to vertebrate peptidic hormones have been searched by immunohistochemical methods in Pecten maximus . Neurosecretory cells have been detected in the nervous ganglia with antisera raised against vertebrate hormones, gastrin/CCK like, glucagon like, insuline like, somatostatine like. Other gastro-intestinal hormones like are localized in some cells of the gut epithelium: bombesine and vasoactive intestinal peptide.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Immunology; Histochemistry; Cells; Neurosecretory system; Juveniles; Hormones.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1724.pdf
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Localization of Sugar Residues in the Stomach of Three Species of Monkeys (Tupaiidae glis, Nycticebus cocang and Callithrix jacchus) by Lectin Histochemistry International Journal of Morphology
Fayed,Masoud Hassan; Elnasharty,Mohamed; Shoaib,Mahmoud.
The stomach of three species of non-human primates was investigated by lectin histochemistry to clarify the staining affinity and distribution patterns of their sugar residues. All gastric regions, with little differences between the deep and superficial parts of the same region, were rich in. in N-acetylglucosamine and/or neuraminic acid. Although, the superficial regions of the gastric mucosa were scanty in N-acetylgalactosamine, a- D-glucose and a -D-mannose, the deep parts of the gastric mucosa were rich in these sugars. In conclusion, there is a difference among the mucosubstances of surface and foveolar mucous cells, mucous neck cells, and gastric gland cells. This indicates heterogeneous composition of gastric mucus, or mucus molecules with...
Tipo: Journal article Palavras-chave: Histochemistry; Lectins; Non-human primates; Stomach.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100016
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Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis) International Journal of Morphology
Kapoor,Kritima; Singh,Opinder.
The present study was conducted on six healthy early neonatal and six prepubertal buffalo calves to study the location, gross morphology, histomorphology and histochemistry of lymphoglandular complexes in proximal colon. In very proximal part of colon of buffalo calves, an irregular oval mucosal lymphoid patch was found grossly as a proximal colon (PC) patch. Histologically, in proximal colon patch of early neonates (3-4 weeks), an extensive invasion of mucosal glands was observed towards lymphoid nodules that were present in submucosa. The structure as a whole thus formed a complex known as lymphoglandular complex (LGC). Large number of such complexes i.e., LGCs were observed in submucosa of proximal colon at this age. At some places, invasion of mucosal...
Tipo: Journal article Palavras-chave: Proximal colon; Lymphoglandular complex; Histomorphology; Histochemistry; Buffalo calves.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300052
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MORPHOANATOMY OF Garcinia madruno (KUNTH) HAMMEL (CLUSIACEAE) UNDER WATERLOGGED CONDITIONS Rev. Bras. Frutic.
ABREU,NATÁLIA DO COUTO; BARBOSA,SUZANA MARQUES; GURGEL,ELY SIMONE CAJUEIRO; CARVALHO,WENDELL VILHENA DE.
ABSTRACT Garcinia madruno (Kunth) Hammel is a neotropical tree that naturally occurs in terra firme forests and is important as a source of income and medicine for Amazonian populations. This study describes and compares the morphoanatomical responses of plants under conditions of stress and normoxia. Young plants of Garcinia madruno were subjected to two water regimes: daily controlled irrigation and waterlogged for 84 days. Hypertrophic lenticels, adventitious roots and anatomical characteristics of the roots and leaves were evaluated on days 0, 21, 42, 63 and 84. There were no changes in leaf structure, but cracks and hypertrophic lenticels appeared on the stems, there was an increase in blackness of the roots and the plants formed adventitious roots to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon; Bacurizinho; Stress; Fruit; Histochemistry.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500201
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Morphoanatomy of nectaries of Chamaecrista (L.) Moench sections Chamaecrista , Caliciopsis and Xerocalyx (Leguminosae: Caesalpinioideae) Acta Botanica
Silva,Marinalva dos Santos; Coutinho,Ítalo Antônio Cotta; Araújo,Maicon Nascimento; Meira,Renata Maria Strozi Alves.
ABSTRACT Nectaries are specialized structures that secrete nectar. Several species of Chamaecrista possess nectaries on the petiole, which have been shown to vary widely in morphology and the chemical nature of their secretion. However, a comprehensive investigation of the nectaries of the clade formed by sect. Chamaecrista, Caliciopsis and Xerocalyx has yet to be performed. Our study aimed to confirm whether or not the leaf glands of species of this clade are in fact nectaries, determine the chemical nature of their secretion and expand the morphoanatomical database on leaf nectaries in Chamaecrista with the intention of contributing to the taxonomy and phylogeny of the genus. Samples from herbarium and field-collected material were subjected to standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Foliar glands; Histochemistry; Leaf glands; Secretory structures; Taxonomy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300445
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Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae) BABT
Moraes,Neci; Scaff,Rejane Cirra.
The objective of our study was to describe by histologic methods the structural organization of the antennal gland of M. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. This study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antennal gland; Macrobrachium potiuna; Morphology; Histochemistry.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300005
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Morphological and histochemical study of the masseter muscle after occlusal alteration Biocell
Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie.
The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p<0.05). Microscopically, C and T groups showed polygonal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003
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Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae Neotropical Entomology
Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A..
The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009
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